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Flow Cytometry

Introduction

The Flow Cytometry facility of IMRB is located at the 1st floor of building R in the Henri Mondor Hospital (how to get to the facility).

It is equipped with one cell sorter (BD Influx Sorp) and two analysers (CyAn ADP LX7 et BD Fortessa X20) to analyze and quantify fluorescence of cells in suspension and to purify defined cells among a population.

Flow cytometry is used for a wide variety of purposes and at IMRB, it is mainly used for cell biology and immune studies.

The Flow Cytometry facility has established tight contacts with the Immunomonitoring Core facility, recently created at IMRB.

Since May 2011, the facility has been awarded an ISO 9001 certification.

Key words

  • flow cytometry
  • cell sorting

Competence and Expertise

  • Scientific and technical fields: cell biology, immunology
  • Competence: Flow cytometry-based cell analyses and sortings

Available Material

INFLUX_pl_tri

Cell sorter : Influx (BD Biosciences)
4 lasers (488, 640, 405 et 561nm)
2 morphological parameters (size and density),
18 fluorescence parameters :
Laser at 488nm : 520/15, 530/40, 670/30 et 710/50.
Laser at 640nm : 670/30, 720/40 et 750LP.
Laser at 405nm : 460/50, 520/35, 610/20, 660/20, 710/50 et 750LP.
Laser at 561nm : 585/29, 610/20, 670/30, 710/50 et 750LP.
Detection of microparticles (<0,2µm).
70 000 events per second.
Up to 6 channels for sorting, 4 nozzle sizes (70, 86, 100 et 140µm).

Monitoring of the temperature  (5°C-95°C)

Collect of purified cells in microtubes, 5 ml, 15 mL or 50 mL tubes.

Possibility of cell cloning in micro-plates (6/24/48/96/384 wells) or on microscopic slides.

 

Analyser: LSRFortessa X20 (BD Biosciences)
3 lasers (405, 488 et 640nm)
2 morphological parameters,
14 fluorescence parameters,
Laser at 488nm : 530/30, 575/26, 610/20, 695/40 et 780/60.
Laser at 640nm : 670/14, 730/45 et 780/60.
Laser at 405nm : 450/50, 525/50, 610/20, 670/30, 710/50 et 780/60.
20 000 events per second.

CyAn_pl_tri

 

Analyser: Cyan, ADP LX7 (Beckman Coulter)
2 lasers (488nm et 633nm)
2 morphological parameters,
7 fluorescence parameters
Laser at 488nm : 530/40, 575/25, 613/20, 680/30, 750LP.
Laser at 633nm : 665/20, 750LP.
70 000 events per second.

 

Low-Jo analysis station (for Mac and PC)

Proposed services

  • Cell sortings
  • Flow cytometry analyses
  • Training for acquisition, analysis and interpretation of data.
  • Provide access of the analyzers after training.
  • Analysis of data using the Flow-Jo software.
  • Advice to users.

Conditions of Use

The facility is opened to all members of IMRB as well as to academic and non-academic teams, provided they obtained agreement from the staff. People and teams willing to use the facility equipment must first meet and discuss with the facility staff in order to establish provisional planning.

Opening hours

From Monday till Friday from 8h30 am to 5h00 pm. Reservation can be made by email at the following address: cytometrie.imrb@inserm.fr. Outside of the opening hours, getting access to the analyzers only requires agreement of the staff.

Contact

Adeline Henry or Aurélie Guguin

IMRB, INSERM U955

Plateforme de Cytométrie en Flux

Hôpital Henri Mondor, Bâtiment Recherche

1er étage, porte AA-064 et AA-061

51, ave du Maréchal de Lattre de Tassigny 94010 Créteil

Tél: 01 49 81 36 03

Mail : cytometrie.imrb@inserm.fr

 

Examples of services provided

Fig-TregAnalysis of human Treg lymphocytes from blood samples


renouvellement_certificatio

 

 

 

 

Since May 9, 2011, the flow cytometry facility is certified  ISO 9001, facilitating its access to industrial partners. Certification has been renewed on April 2014.
ISO 9001 certification defines a set of requirements concerning the implementation of a quality management system, regardless of size and industrial activity.